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Welcome to the PacBio training page. Here, you can search for and download online webinars, e-Modules, and other training documents. If you have any questions about our training materials, please contact us.

30 8 月, 2024

Technical overview – Kinnex library preparation using Kinnex single-cell RNA kit

  • Tutorial
  • Revio system, Sequel II System:Sequel IIe System
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of Kinnex library preparation using the Kinnex single-cell RNA kit. Sequencing preparation workflow details for Kinnex single-cell RNA libraries as well as example sequencing performance data are also presented.
30 8 月, 2024

Technical overview — Kinnex kits for single-cell RNA and full-length RNA and 16S rRNA sequencing

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides a general overview of Kinnex library preparation using the Kinnex single-cell RNA kit, Kinnex full-length RNA kit and the Kinnex 16S rRNA kit. Sequencing preparation workflow details for Kinnex libraries as well as example sequencing performance data are also presented.
30 8 月, 2024

Technical overview — Kinnex library preparation using Kinnex full-length RNA kit

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of Kinnex library preparation using the Kinnex full-length RNA kit. Sequencing preparation workflow details for Kinnex full-length RNA libraries as well as example sequencing performance data are also presented.
30 8 月, 2024

Technical overview — Kinnex library preparation for full-length 16S rRNA sequencing

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of Kinnex library preparation for full-length 16S rRNA gene sequencing. Sequencing preparation workflow details for Kinnex 16S rRNA libraries as well as example sequencing performance data are also presented.
19 4 月, 2024

Technical overview – Whole genome and metagenome library preparation using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation describes sample preparation details for constructing whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using SMRTbell prep kit 3.0 (SPK 3.0) for sequencing on PacBio Sequel II and Sequel IIe systems. Options for multiplexing WGS samples using SMRTbell barcoded adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations for de novo assembly, variant detection and shotgun metagenomics are also presented.
4 4 月, 2024

Technical overview — HiFi library preparation using HiFi prep kits for high-throughput sequencing on PacBio long-read systems

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of HiFi library preparation using HiFi prep kit 96 and HiFi plex prep kit 96. Sequencing preparation workflow details for HiFi prep kit libraries as well as example sequencing performance data are also presented.
25 3 月, 2024

Technical overview — Automated high-throughput HMW DNA extraction for PacBio long-read sequencing using Nanobind HT kits

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation

This technical training presentation describes Nanobind HT HMW DNA extraction kits for enabling high-throughput DNA sample preparation automation on four robotic platforms: Hamilton Microlab NIMBUS Presto, Thermo Fisher KingFisher Apex, Flex & Duo Prime systems. Example HiFi sequencing performance data for PacBio SMRTbell libraries constructed from Nanobind HT-extracted DNA are also presented.
25 3 月, 2024

Technical overview — HMW DNA sample preparation for PacBio long-read sequencing using Nanobind PanDNA and SRE kits

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation

This technical training presentation describes the use of Nanobind kits for high-molecular weight (HMW) DNA extraction from a wide range of sample types including cultured cells, cultured bacteria (gram-negative and gram-positive), whole blood (nucleated, non-nucleated, and red blood cell lysis), diverse animal tissues, insects, and plant nuclei. Example HiFi sequencing performance data for PacBio SMRTbell libraries constructed from Nanobind-extracted DNA are also presented.
25 3 月, 2024

Technical overview – PureTarget repeat expansion panel library preparation using PureTarget kit

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of PureTarget library preparation for PureTarget repeat expansion panel sequencing. Sequencing preparation workflow details for PureTarget repeat expansion panel libraries as well as example sequencing performance data are also presented.
9 2 月, 2024

Technical overview — Revio ICS v13 and SMRT Link v13

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of key features & benefits of the new Revio system ICS v13 and SMRT Link v13 release.
13 6 月, 2023

Technical overview – MAS-Seq library preparation using the MAS-Seq for 10x Single Cell 3’ kit

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of multiplexed arrays sequencing (MAS-Seq) library preparation using the using the MAS-Seq for 10x Single Cell 3’ kit. Sequencing preparation workflow details, example sequencing performance data, and single-cell MAS-Seq data analysis recommendations are also presented.
5 4 月, 2023

Technical overview Nanobind HT kits for automated HMW DNA extraction

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation

This technical training presentation describes Nanobind HT HMW DNA extraction kits for enabling high-throughput DNA sample preparation automation on four robotic platforms: Hamilton Microlab NIMBUS Presto, Thermo Fisher KingFisher Apex, Flex & Duo Prime systems. Example HiFi sequencing performance data for PacBio SMRTbell libraries constructed from Nanobind HT-extracted DNA are also presented.
7 11 月, 2022

Technical overview – Multiplexed amplicon library preparation using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II System:Sequel IIe System, Revio system
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of multiplexed amplicon library sample preparation using SMRTbell prep kit 3.0 (SPK 3.0) in conjunction with barcoded primers, SMRTbell barcoded adapters or barcoded M13 primers. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations are also presented. Additional guidance for preparing multiplexed amplicon libraries for full-length 16S amplicon sequencing, Human Leukocyte Antigen (HLA) sequencing and HiFi target capture enrichment sequencing (using Twist Bioscience panels) is also summarized.
20 4 月, 2022

Technical overview – Adeno-associated virus (AAV) library preparation using SMRTbell Prep Kit 3.0

  • Tutorial
  • Sequel II System:Sequel IIe System, Sequel II System, Sequel IIe System
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of adeno-associated virus (AAV) library preparation for viral genome sequencing using SMRTbell prep kit 3.0 (SPK 3.0). Options for multiplexing AAV samples using SMRTbell barcoded adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and HiFiViral SARS-CoV-2 data analysis recommendations are also presented.
20 4 月, 2022

Technical overview – Iso-Seq library preparation using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II System, Sequel IIe System, Sequel II System:Sequel IIe System
  • Sample Preparation, Library Preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of Iso-Seq library sample preparation using SMRTbell prep kit 3.0 (SPK 3.0). Options for multiplexing Iso-Seq samples using barcoded cDNA primers or SMRTbell barcoded adapters are discussed. Sequencing preparation workflow details, example performance data and Iso-Seq data analysis recommendations are also presented.
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