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Featured Documentation

This Featured Documentation page provides the latest and most frequently used guides, protocols, and product information. See Other Documentation for more specialized and older documentation.

4月 24, 2023

SMRT Link User Guide v12.0

  • Informational Guide
  • Sequel II System:Sequel IIe System, Revio system
  • Sequencing, Analysis

Description

This document describes how to use PacBio’s SMRT Link software. SMRT Link is the web-based end-to-end workflow manager for RevioTM, Sequel® II, and Sequel IIe systems.
3月 8, 2023

Operations guide – Revio system

  • Guide
  • Revio system
  • Sequencing

Description

This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT Link (v12.0) user guide.
3月 8, 2023

SMRT Tools Reference Guide v12.0

  • Developer Documentation
  • Sequel II System:Sequel IIe System, Revio system
  • Sequencing, Analysis

Description

This document describes the command-line tools included with SMRT® Link v12.0. These tools are for use by bioinformaticians working with secondary analysis results.
10月 10, 2022

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

  • Procedure & Checklist
  • Sequel II System, Sequel IIe System
  • Library Preparation

Description

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.
9月 18, 2023

Release notes – Revio system instrument control software v12.0.4

  • Release Notes
  • Revio system
  • Sequencing

Description

The Revio system v12.0.4 Instrument Control Software (ICS) patch release provides the same run design options and formats, primary and post-primary analysis algorithms, and output file content and formats as previous Revio system v12.0 ICS versions.
9月 9, 2023

Quick reference card — Onso system library preparation adapters

  • Quick Reference Card
  • Onso system
  • Library Preparation

Description

Onso indexed adapter sequences are shown here and can be purchased to prepare libraries from genomic, fragmented, or existing P5/P7 libraries. See the appropriate procedures and user guide for more information.
9月 9, 2023

Safety guide — Revio system

  • Guide & Overview
  • Revio system
  • Sequencing

Description

This guide provides important safety information on the Revio system from Pacific Biosciences. It also provides product compliance and regulatory statements. Read this document before performing any procedures on the Revio systems.
8月 31, 2023

SMRT Link Installation v12.0

  • Informational Guide
  • Sequel II System:Sequel IIe System, Revio system
  • Sequencing, Analysis

Description

This document describes the procedure for installing SMRT Link v12.0. This document is for Customer IT or SMRT Link administrators. SMRT Link v12.0 supports RevioTM systems, Sequel® II systems and Sequel IIe systems. Sequel systems are not supported.
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