Featured Documentation

SMRT Link Kinnex single-cell troubleshooting guide v13.1

Description

This document describes the metrics generated by the Read Segmentation and Single-Cell Iso-Seq® workflow in SMRT Link v13.1. The document also describes possible issues that can occur when using the Kinnex single-cell RNA kit for both supported and unsupported use cases.

SMRT Tools Reference Guide v13.1

Description

This document describes the command-line tools included with SMRT Link v13.1

Revio run evaluation and troubleshooting guide

Description

Run evaluation and troubleshooting guide for the Revio system

SMRT Link Release Notes v13.1

Description

Release notes for SMRT Link v13.1

SMRT Link Installation Guide v13.1

Description

Installation guide for SMRT Link v13.1

Release notes — Revio system instrument control software v13.1

Description

Release notes for the Revio system Instrument Control Software v13.1

SMRT Link User Guide v13.1

Description

This document describes how to use SMRT Link software from PacBio. SMRT Link is the web-based end-to-end workflow manager for Revio, Sequel II, and Sequel IIe systems.

Procedure & checklist – Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

SMRT Link User Guide v13.0

Description

This document describes how to use SMRT Link software from PacBio. SMRT Link is the web-based end-to-end workflow manager for Revio, Sequel II, and Sequel IIe systems.

Release notes — Revio system ICS v13.0-0-212033

Description

The Revio system Instrument Control Software (Revio ICS) v13.0.0.212033 release notes

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio® Revio™, Sequel® II, and IIe systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters.

Overview – HiFi application options

Description

Overview of HiFi application options

Operations guide – Revio system

Description

This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT Link (v12.0) user guide.

Guide & overview – Nanobind CBB kit

Description

This document describes procedures on how to use the Nanobind CBB Kit for extraction of HMW (50 kb – 300+ kb) DNA from cultured cells, cultured bacteria, and blood.

Guide & overview — Nanobind PanDNA kit

Description

This guide and overview describes the PacBio® Nanobind PanDNA kit which can be used for diverse sample types including cultured cells and bacteria, human blood and tissue, animal blood and tissue, insects, and plant nuclei. For each sample type, a detailed Procedure & Checklist can be found at the PacBio Documentation page.