Featured Documentation

Overview – HiFi application options

Description

Overview of HiFi application options

SMRT Link User Guide v12.0

Description

This document describes how to use PacBio’s SMRT Link software. SMRT Link is the web-based end-to-end workflow manager for RevioTM, Sequel® II, and Sequel IIe systems.

Procedure & checklist – Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Operations guide – Revio system

Description

This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT Link (v12.0) user guide.

SMRT Link Release Notes v12.0

SMRT Tools Reference Guide v12.0

Description

This document describes the command-line tools included with SMRT® Link v12.0. These tools are for use by bioinformaticians working with secondary analysis results.

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.

Release notes – Revio system instrument control software v12.0.4

Description

The Revio system v12.0.4 Instrument Control Software (ICS) patch release provides the same run design options and formats, primary and post-primary analysis algorithms, and output file content and formats as previous Revio system v12.0 ICS versions.

Quick reference card — Onso system library preparation adapters

Description

Onso indexed adapter sequences are shown here and can be purchased to prepare libraries from genomic, fragmented, or existing P5/P7 libraries. See the appropriate procedures and user guide for more information.

Safety guide — Revio system

Description

This guide provides important safety information on the Revio system from Pacific Biosciences. It also provides product compliance and regulatory statements. Read this document before performing any procedures on the Revio systems.

SMRT Link Installation v12.0

Description

This document describes the procedure for installing SMRT Link v12.0. This document is for Customer IT or SMRT Link administrators. SMRT Link v12.0 supports RevioTM systems, Sequel® II systems and Sequel IIe systems. Sequel systems are not supported.

Operations Guide — Onso system cluster generator and short-read sequencer

Description

This guide describes the workflow for preparing Sequencing By Binding libraries for cluster generation on the PacBio Onso cluster generator system, as well as preparation for and sequencing of clustered flow cells on the Onso system.

Procedure & checklist Preparing Onso libraries from existing P5P7 libraries for short-read sequencing

Description

This procedure describes the workflow for converting existing P5/P7-tailed libraries to be compatible for sequencing on PacBio short-read sequencing systems.

Procedure & checklist – Generating hybrid capture libraries for sequencing on the Onso system

Description

This procedure describes how the Onso DNA Library prep kit (PacBio 102-431-400), Onso Library amp kit (PacBio 102-410-800) and Onso Blocker oligos (PacBio 102-427-200) can be used to support the generation of hybrid capture libraries compatible with sequencing on PacBio short-read sequencing systems.

Procedure & checklist – Preparing Onso libraries from fragmented DNA for short-read sequencing

Description

This procedure describes the workflow for constructing Onso libraries from fragmented genomic material for sequencing on PacBio short-read sequencing systems.