Application Brochures and Briefs Archives - 第2页共2页

2021年08月19日 | 测序方法

Application Brief: Targeted sequencing for amplicons – Best Practices

With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel System, you can easily and cost effectively generate highly accurate long reads (HiFi reads, >99% single-molecule accuracy) from genes or regions of interest ranging in size from several hundred base pairs to 20 kb. Target all types of variation across relevant genomic regions, including low complexity regions like repeat expansions, promoters, and flanking regions of transposable elements.

2021年08月19日 | 测序方法

Application Brief: Long-read RNA sequencing – Best Practices

With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel Systems, you can easily and affordably sequence complete transcript isoforms in genes of interest or across the entire transcriptome. The Iso-Seq method allows users to generate full-length cDNA sequences up to 10 kb in length — with no assembly required — to confidently characterize full-length transcript isoforms.

2021年08月19日 |

Application Brochure: HiFi reads for highly accurate long-read sequencing

Discover the benefits of HiFi reads and learn how highly accurate long-read sequencing provides a single technology solution across a range of applications.

2021年08月19日 | 测序方法

Application Brief: Single-cell RNA sequencing with HiFi reads – Best Practices

With PacBio single-cell RNA sequencing using the Iso-Seq method, you can now distinguish between alternative transcript isoforms at the single-cell level. The highly accurate long reads (HiFi reads) can span the entire 5′ to 3′ end of a transcript, allowing a high-resolution view of isoform diversity and revealing cell-to-cell heterogeneity without the need for assembly.

2021年08月19日 | 人类遗传学研究

Application Brochure: Gene editing validation with HiFi reads

With highly accurate long reads (HiFi reads) from the Sequel IIe System, powered by Single Molecule, Real-Time (SMRT) Sequencing technology, you can efficiently and cost effectively validate gene editing techniques including adeno-associated virus (AAV) and CRISPR-Cas9 approaches.

2021年08月19日 | 测序方法

Application Brochure: Scalable human whole genome HiFi sequencing for rare and inherited disease research

PacBio highly accurate long reads – HiFi reads – offer a single-platform solution for rare and inherited disease research, elucidating suspected genetic causes of disease in up to ~50% of cases that have not previously been explained using short-read exome or whole genome sequencing. PacBio offers an efficient workflow, developed in collaboration with Children’s Mercy Kansas City, which provides a scalable solution for sequencing 100s to 1000s of whole human genomes per year on the Sequel II and Sequel IIe Systems.

2021年08月19日 | 测序方法

Application Brief: Whole genome sequencing for de novo assembly – Best Practices

PacBio HiFi reads provide both long read lengths (up to 25 kb) and high accuracy (>99.9%) to quickly and affordably generate contiguous, complete, and correct de novo genome assemblies of even the most complex genomes.

2021年08月19日 | 宏基因组学

Application Brief: Metagenomic sequencing with HiFi reads – Best Practices

Highly accurate long reads – HiFi reads – with single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, shotgun metagenomic profiling, and metagenome assembly.

2021年08月19日 | 公共卫生监测

Application Brief: Variant detection using whole genome sequencing with HiFi reads – Best Practices

With highly accurate long reads (HiFi reads) from the Sequel II or IIe Systems you can comprehensively detect variants in 100s to 1000s of genomes in a year. HiFi reads provide high precision and recall for single nucleotide variants (SNVs), indels, structural variants (SVs), and copy number variants (CNVs), including in difficult-to-map repetitive regions.

Asset Type： Application Brochures and Briefs

Application Brief: Targeted sequencing for amplicons – Best Practices

Application Brief: Long-read RNA sequencing – Best Practices

Application Brochure: HiFi reads for highly accurate long-read sequencing

Application Brief: Single-cell RNA sequencing with HiFi reads – Best Practices

Application Brochure: Gene editing validation with HiFi reads

Application Brochure: Scalable human whole genome HiFi sequencing for rare and inherited disease research

Application Brief: Whole genome sequencing for de novo assembly – Best Practices

Application Brief: Metagenomic sequencing with HiFi reads – Best Practices

Application Brief: Variant detection using whole genome sequencing with HiFi reads – Best Practices

订阅博客

Filter by topic

咨询专家

ALS 案例研究

订阅博客

Filter by topic

咨询专家