Asset Type： Application Brochures and Briefs
2023年12月26日 | PacBio新闻
Application brief – HiFi sequencing enables greater accuracy of somatic variant calling
2023年10月19日 | procedures + protocols 产品、程序+实验方案
Application brief — Germline variant detection with the Onso system
2023年08月26日 | procedures + protocols 产品、程序+实验方案
Application brief — What can you do with a Revio system
2022年06月09日 | 样本+文库制备
Application-Brief — HiFi-Target-Enrichment-Best-Practices
With HiFi target enrichment you can easily and cost-effectively sequence just the genes you care about at scale. Design custom gene panels with our partners at Twist, sequence them with long and accurate HiFi reads, and get all the benefits you expect from HiFi reads: comprehensive detection of single nucleotide variants, structural variants, and indels. HiFi target enrichment is the only technology that can resolve complex gene families like HLA or Cytochrome P450 genes with haplotype resolution.
2022年05月26日 | 产品、程序+实验方案
HIFI WGS at scale on the Sequel IIe system
Scale your whole genome sequencing (WGS) and epigenome workflows with PacBio® HiFi reads employing a single technician working one day a week. Sequence 25 human genomes at ≥ 30-fold coverage each month using four Sequel® II systems.*
2022年05月16日 | AAV
Application Brochure AAV Sequencing with HiFi Reads
With Single Molecule, Real-Time (SMRT®) sequencing on the Sequel® IIe systems, you can sequence AAV
genome populations to identify truncation, mutation, and host integration events. The AAV workflow from
PacBio® accommodates both scAAV and ssAAV constructs with easy on-instrument HiFi read generation
2022年04月20日 | 产品、程序+实验方案
Application Brief – Measuring DNA methylation with 5-base HiFi sequencing
HiFi sequencing produces long, accurate reads of the 4 DNA bases — A, C, G, and T — that deliver the most comprehensive characterization of genomes. But HiFi sequencing is not limited to characterizing the genome. It simultaneously measures the epigenome by detecting a fifth base — 5mC at CpG sites — without requiring special library preparation like bisulfite treatment. This detects distinct regional epigenetic patterns, accesses methylation in the full genome, and identifies allele-specific methylation.
2022年04月07日 | 抗肿瘤研究
Application Brief: Uncover Cancer-Specific RNA Isoforms Using Long Read Sequencing
The Iso-Seq method utilizes long-read transcript sequencing to reliably capture full-length transcript isoforms without the need for
computational assembly. PacBio’s long-read RNA sequencing is also significantly less error-prone than other long-read sequencing
technologies, offering more robust and accurate isoform discovery power.9,10 Future research will continue to explore the complex and
mostly unexplored landscape of isoforms across cancer types.
2021年11月15日 | 传染病研究
Application Brief: Microbial whole genome sequencing – Best Practices
With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel Systems, you can affordably assemble reference-quality microbial genomes that are >99.999% (Q50) accurate.
2021年11月15日 |
Application Brochure: What can you do with one SMRT Cell?
With PacBio Single Molecule, Real-Time (SMRT) Sequencing on the Sequel IIe System you can characterize whole genomes and transcriptomes with just one SMRT Cell. Explore our applications and pricing to get your sequencing project started.
2021年11月15日 | 传染病研究
Application Brief: HiFiViral SARS-CoV-2 for COVID-19 whole genome sequencing – Best Practices
HiFiViral for SARS-CoV-2 is a simple-to-use, scalable, cost-effective solution for sequencing the entire SARS-CoV-2 genome. This fully kitted solution uses a novel approach that is robust to new variants and comprehensively detects all types of mutations.
2021年08月19日 | 抗肿瘤研究
Cancer Brochure: Sequence cancer variants with confidence
To bring personalized medicine to all patients, cancer researchers need more reliable and comprehensive views of somatic variants of all sizes that drive cancer biology.
2021年08月19日 | 测序方法
Application Brief: Targeted sequencing for amplicons – Best Practices
With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel System, you can easily and cost effectively generate highly accurate long reads (HiFi reads, >99% single-molecule accuracy) from genes or regions of interest ranging in size from several hundred base pairs to 20 kb. Target all types of variation across relevant genomic regions, including low complexity regions like repeat expansions, promoters, and flanking regions of transposable elements.
2021年08月19日 | 测序方法
Application Brief: Long-read RNA sequencing – Best Practices
With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel Systems, you can easily and affordably sequence complete transcript isoforms in genes of interest or across the entire transcriptome. The Iso-Seq method allows users to generate full-length cDNA sequences up to 10 kb in length — with no assembly required — to confidently characterize full-length transcript isoforms.
2021年08月19日 |
Application Brochure: HiFi reads for highly accurate long-read sequencing
Discover the benefits of HiFi reads and learn how highly accurate long-read sequencing provides a single technology solution across a range of applications.
