PacBio Literature

HiFi metagenome profiling provides more (and richer) functional information and precise species classifications. Each HiFi read typically has an average of eight intact genes per read, providing richer information about genes that co-occur in a single microbe, and may be co-expressed. 80-90% of HiFi reads have at least one functional annotation, and typically average four, whereas only ~1/3 of short reads can be annotated even once. HiFi reads can assign taxonomy with high precision and recall

Accurate and flexible HiFi solutions for metagenomics and microbiome sequencing allow researchers to study microbes and microbial communities in high resolution without the need for culturing.

Detect, track, and characterize pathogens to a new standard of completeness and precision. PacBio® solutions offer unmatched accuracy and long read lengths to support a wide range of pathogen surveillance applications.

PacBio in collaboration with Covaris has developed a workflow that enables extraction, preparation, and sequencing of FFPE-derived DNA for HiFi sequencing.

Application brief — What can you do with one SMRT Cell

Highly accurate long- and short-read sequencing from PacBio® allows cancer researchers to characterize the complete spectrum of cancer variation in a single assay, yielding a clearer view of its biology, helping to identify new therapeutic targets and inform clinical decision making.

Microbial genomics with PacBio long-read HiFi sequencing is accurate, scalable, and cost effective. Automated end-to-end workflows from extraction to analysis and optimized kits drive greater throughput for HiFi microbial sequencing at lower cost.

Highly accurate long reads – HiFi reads – with single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, shotgun metagenomic profiling, and metagenome assembly.

With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel Systems, you can affordably assemble reference-quality microbial genomes that are >99.999% (Q50) accurate.

CiFi (pronounced “Sci-Fi”) is a new application developed by the sequencing community that integrates chromatin conformation capture (3C) with PacBio HiFi long-read sequencing to generate highly accurate, multi-contact reads.

This Application note provides the workflow for the detection of somatic small variants, structural variants, and methylation for paired tumor-normal samples with HiFi whole genome sequencing (WGS)

Explore how HiFi sequencing can be used to support your gene and cell therapy research

This technical note describes the experimental conditions of a modified workflow preparing PacBio Kinnex single-cell libraries with Twist exome enrichment kits.

Powered by the exceptional accuracy of HiFi sequencing and the Tandem Repeat Genotyping Tool (TRGT), the PacBio® PureTarget™ repeat expansion panel offers more comprehensive genotyping for 20 of the most important repeat expansions for human health. This application note demonstrates the performance of the PureTarget repeat expansion panel, and presents the PureTarget panel as a scalable, more comprehensive solution for profiling repeat expansions, compared to legacy genotyping and next-generation sequencing methods.

This application note demonstrates that DNA extracted from whole blood and saliva samples using the Nanobind kits yields high-quality HiFi sequencing data.