PacBio Literature

This technical note describes one set of experimental conditions for shearing high molecular weight genomic DNA (gDNA) derived from human whole blood using MP Biomedicals’ FastPrep-96 instrument. These results demonstrate the FastPrep-96 as a high-throughput DNA shearing option for WGS.

Powered by the innovative sequencing by binding (SBB®) technology, the Onso system delivers unprecedented accuracy and sensitivity to advance your critical and groundbreaking research.

PacBio® HiFi long-read amplicon sequencing has demonstrated advantages for high-throughput, high- resolution eukaryotic barcode sequencing.

The Onso library preparation kits are used to create libraries for the PacBio Onso short-read sequencing system.

The outstanding accuracy of HiFi long-read sequencing allows you to get the most out of each Revio run. Discover how you can access more information at reduced cost and higher throughput for each of your sequencing projects.

The Onso short-read sequencing system is a scalable and flexible benchtop platform that provides an extraordinary level of accuracy through its unique sequencing by binding (SBB™) technology.

The Onso system, powered by the extraordinary accuracy of sequencing by binding chemistry, now enables the sensitivity necessary to detect low-frequency variants in liquid biopsy applications.

Detect, track, and characterize pathogens to a new standard of completeness and precision. PacBio® solutions offer unmatched accuracy and long read lengths to support a wide range of pathogen surveillance applications.

With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel Systems, you can affordably assemble reference-quality microbial genomes that are >99.999% (Q50) accurate.

In this whitepaper, we discuss the features of bulk and single-cell isoform sequencing with the Iso-Seq method and their applications in human genomics.

HiFi reads can span most repeats, making it possible to comprehensively profile variation in repeat regions and to measure any changes in methylation. PacBio has developed the Tandem repeat genotyping tool (TRGT) and Tandem repeat visualizer (TRVZ) as bioinformatics tools to accurately profile short tandem repeats, variable number tandem repeats, and macrosatellites.

Plate-based shearing using the SPEX SamplePrep 1600 MiniG homogenizer is a high-throughput and cost-effective alternative to the standard, lower-throughput options recommended for HiFi sequencing on PacBio long-read systems

HiFi metagenome profiling provides more (and richer) functional information and precise species classifications. Each HiFi read typically has an average of eight intact genes per read, providing richer information about genes that co-occur in a single microbe, and may be co-expressed. 80-90% of HiFi reads have at least one functional annotation, and typically average four, whereas only ~1/3 of short reads can be annotated even once. HiFi reads can assign taxonomy with high precision and recall

Accurate and flexible HiFi solutions for metagenomics and microbiome sequencing allow researchers to study microbes and microbial communities in high resolution without the need for culturing.

Partnerships with purpose. The PacBio® Compatible program unites a wide range of industry-leading organizations with the exceptional accuracy of PacBio sequencing workflows.