PacBio Literature

Application brief — What can you do with one SMRT Cell

Explore how HiFi sequencing can be used to support your gene and cell therapy research

HiFi systems brochure

Unravel the complexity of plant and animal biology for any species with HiFi sequencing. Discover more about species and populations with more accurate genomes, transcriptomes, and epigenomes.

Unravel the complexity of plant and animal genomes with HiFi sequencing to harness agrigenomics insights for breeding, crop protection, and genome engineering.

PacBio® provides cutting-edge sequencing that allows scientists to unlock the genetic diversity of all species on earth. These tools can fuel discoveries in agriculture, biodiversity, and environmental health, and build toward a sustainable future.

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

See it all with HiFi sequencing on the Revio™ system

HiFi metagenome profiling provides more (and richer) functional information and precise species classifications. Each HiFi read typically has an average of eight intact genes per read, providing richer information about genes that co-occur in a single microbe, and may be co-expressed. 80-90% of HiFi reads have at least one functional annotation, and typically average four, whereas only ~1/3 of short reads can be annotated even once. HiFi reads can assign taxonomy with high precision and recall

Accurate and flexible HiFi solutions for metagenomics and microbiome sequencing allow researchers to study microbes and microbial communities in high resolution without the need for culturing.

Detect, track, and characterize pathogens to a new standard of completeness and precision. PacBio® solutions offer unmatched accuracy and long read lengths to support a wide range of pathogen surveillance applications.

PacBio in collaboration with Covaris has developed a workflow that enables extraction, preparation, and sequencing of FFPE-derived DNA for HiFi sequencing.

Highly accurate long- and short-read sequencing from PacBio® allows cancer researchers to characterize the complete spectrum of cancer variation in a single assay, yielding a clearer view of its biology, helping to identify new therapeutic targets and inform clinical decision making.

Microbial genomics with PacBio long-read HiFi sequencing is accurate, scalable, and cost effective. Automated end-to-end workflows from extraction to analysis and optimized kits drive greater throughput for HiFi microbial sequencing at lower cost.

Highly accurate long reads – HiFi reads – with single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, shotgun metagenomic profiling, and metagenome assembly.