PacBio Literature

Explore how HiFi sequencing can be used to support your gene and cell therapy research

The Vega system is an innovative Revio technology, reimagined in a benchtop solution with seamless integration with HiFi library prep workflows and SMRT Link run management software. On-instrument primary analysis, including DeepConsensus, methylation calling, and optional barcode demultiplexing in every run. SPRQ-Nx chemistry enables lower cost per run, 90 Gb yield, single-shift movies, and supports 21 CFR Part 11 compliance.

With highly accurate long reads (HiFi reads), powered by Single Molecule, Real-Time (SMRT) sequencing technology, you can more comprehensively validate gene editing techniques such as CRISPR-Cas9 approaches.

With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel System, you can easily and cost effectively generate highly accurate long reads (HiFi reads, >99% single-molecule accuracy) from genes or regions of interest ranging in size from several hundred base pairs to 20 kb. Target all types of variation across relevant genomic regions, including low complexity regions like repeat expansions, promoters, and flanking regions of transposable elements.

A streamlined targeted sequencing approach for existing PCR assays is to use indexed SMRTbell adapters. With the automated HiFi plex prep kit, prepare individual SMRTbell libraries for each sample, pool, and sequence on one SMRT Cell.

Application brief — What can you do with one SMRT Cell

HiFi systems brochure

Unravel the complexity of plant and animal biology for any species with HiFi sequencing. Discover more about species and populations with more accurate genomes, transcriptomes, and epigenomes.

Unravel the complexity of plant and animal genomes with HiFi sequencing to harness agrigenomics insights for breeding, crop protection, and genome engineering.

PacBio® provides cutting-edge sequencing that allows scientists to unlock the genetic diversity of all species on earth. These tools can fuel discoveries in agriculture, biodiversity, and environmental health, and build toward a sustainable future.

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

See it all with HiFi sequencing on the Revio™ system

HiFi metagenome profiling provides more (and richer) functional information and precise species classifications. Each HiFi read typically has an average of eight intact genes per read, providing richer information about genes that co-occur in a single microbe, and may be co-expressed. 80-90% of HiFi reads have at least one functional annotation, and typically average four, whereas only ~1/3 of short reads can be annotated even once. HiFi reads can assign taxonomy with high precision and recall

Accurate and flexible HiFi solutions for metagenomics and microbiome sequencing allow researchers to study microbes and microbial communities in high resolution without the need for culturing.

Detect, track, and characterize pathogens to a new standard of completeness and precision. PacBio® solutions offer unmatched accuracy and long read lengths to support a wide range of pathogen surveillance applications.