PacBio Literature

This Application note provides the workflow for the detection of somatic small variants, structural variants, and methylation for paired tumor-normal samples with HiFi whole genome sequencing (WGS)

Kinnex offers full-length isoform discovery and abundance information, cell type-specific isoform discovery, and species-level metagenomics community identification that are difficult to capture with short reads.

SMRT® Link software tools for PacBio® HiFi sequencing systems are designed to set up and monitor sequencing runs, review performance metrics, and analyze, visualize, and annotate sequencing data. SMRT Link is available for both local installation and cloud access, offering flexibility to work in different environments depending on your lab’s needs.

Highly accurate long- and short-read sequencing from PacBio® allows cancer researchers to characterize the complete spectrum of cancer variation in a single assay, yielding a clearer view of its biology, helping to identify new therapeutic targets and inform clinical decision making.

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

Vega system specification sheet

See it all with HiFi sequencing on the Revio™ system

Vega benchtop system brochure

HiFi systems brochure

Highlighting publications using Iso-Seq in cancer research to demonstrate how bulk and single-cell Iso- Seq data detects fusion genes, identifies novel isoforms that might serve as neoepitopes for vaccine candidates, and traces clonal evolution.

This technical note describes the experimental conditions of a modified workflow preparing PacBio Kinnex single-cell libraries with Twist exome enrichment kits.

Reveal the complexity of the human genome to power human disease research — With the advancement of highly accurate long-read sequencing, missing pieces could be resolved and fully complete the genome. PacBio HiFi sequencing gave rise to this achievement and has dramatically improved understanding of the human genome by resolving gaps that are unattainable by other sequencing methods, accurately mapping complex regions, and enabling more contiguous genome assemblies.

Sequencing has become an integral part of the biopharma research and development process and enhances our understanding of the molecular determinants of health and disease. Whether you are conducting research on novel potential drug targets, biomarkers, or therapeutics such as gene therapies, PacBio® offers highly accurate sequencing solutions across its portfolio that can improve your chances of success and may reduce your time to market.

This technical note describes the procedure for targeted enrichment using IDT xGen Hyb and Wash kit v3 for Kinnex™ full-length RNA libraries.

Unravel the complexity of plant and animal biology for any species with HiFi sequencing. Discover more about species and populations with more accurate genomes, transcriptomes, and epigenomes.