PacBio Literature

Highly accurate long- and short-read sequencing from PacBio® allows cancer researchers to characterize the complete spectrum of cancer variation in a single assay, yielding a clearer view of its biology, helping to identify new therapeutic targets and inform clinical decision making.

Microbial genomics with PacBio long-read HiFi sequencing is accurate, scalable, and cost effective. Automated end-to-end workflows from extraction to analysis and optimized kits drive greater throughput for HiFi microbial sequencing at lower cost.

Highly accurate long reads – HiFi reads – with single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, shotgun metagenomic profiling, and metagenome assembly.

With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel Systems, you can affordably assemble reference-quality microbial genomes that are >99.999% (Q50) accurate.

CiFi (pronounced “Sci-Fi”) is a new application developed by the sequencing community that integrates chromatin conformation capture (3C) with PacBio HiFi long-read sequencing to generate highly accurate, multi-contact reads.

This Application note provides the workflow for the detection of somatic small variants, structural variants, and methylation for paired tumor-normal samples with HiFi whole genome sequencing (WGS)

Explore how HiFi sequencing can be used to support your gene and cell therapy research

This technical note describes the experimental conditions of a modified workflow preparing PacBio Kinnex single-cell libraries with Twist exome enrichment kits.

Powered by the exceptional accuracy of HiFi sequencing and the Tandem Repeat Genotyping Tool (TRGT), the PacBio® PureTarget™ repeat expansion panel offers more comprehensive genotyping for 20 of the most important repeat expansions for human health. This application note demonstrates the performance of the PureTarget repeat expansion panel, and presents the PureTarget panel as a scalable, more comprehensive solution for profiling repeat expansions, compared to legacy genotyping and next-generation sequencing methods.

This application note demonstrates that DNA extracted from whole blood and saliva samples using the Nanobind kits yields high-quality HiFi sequencing data.

The Kinnex single-cell RNA kit is compatible with cDNA generated using the Visium HD 3' Spatial Gene Expression assay for creating a sequencing-ready library that can run on PacBio sequencing systems

Unravel the complexity of plant and animal genomes with HiFi sequencing to harness agrigenomics insights for breeding, crop protection, and genome engineering.

In this whitepaper, we discuss the features of full- length and single-cell isoform sequencing with the Iso-Seq method and their applications in plant and animal research. We show how combining the Iso-Seq method with Kinnex kits further increases throughput, making PacBio long reads a cost-effective RNA sequencing solution.

Unravel the complexity of plant and animal biology for any species with HiFi sequencing. Discover more about species and populations with more accurate genomes, transcriptomes, and epigenomes.

PacBio® provides cutting-edge sequencing that allows scientists to unlock the genetic diversity of all species on earth. These tools can fuel discoveries in agriculture, biodiversity, and environmental health, and build toward a sustainable future.