PacBio Literature

HiFi systems brochure

Vega system specification sheet

The Kinnex single-cell RNA kit takes as input single- cell cDNA and outputs a sequencing-ready library that results in a 16-fold throughput increase compared to regular single-cell Iso-Seq libraries.

Powered by the innovative sequencing by binding (SBB®) technology, the Onso system delivers unprecedented accuracy and sensitivity to advance your critical and groundbreaking research.

This technical note describes the experimental conditions of a modified workflow preparing PacBio Kinnex single-cell libraries with Twist exome enrichment kits.

Microbial genomics with PacBio long-read HiFi sequencing is accurate, scalable, and cost effective. Automated end-to-end workflows from extraction to analysis and optimized kits drive greater throughput for HiFi microbial sequencing at lower cost.

Highly accurate long reads – HiFi reads – with single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, shotgun metagenomic profiling, and metagenome assembly.

PureTarget offers an easy and scalable workflow to capture repeat expansions and brings you from sample to answer in three days. Compatible with the RevioTM and Sequel® II systems, PureTarget libraries can be multiplexed with up to 48 samples, generating deep coverage across the 20-gene target panel.

PacBio long-read HiFi sequencing allows you to sequence the entire AAV genome with extraordinary accuracy and completeness so that you can quickly discover and optimize gene therapy vectors.

PacBio long-read sequencing technology and Form Bio’s streamlined data analysis now offer an all-in-one solution to optimize your AAV vector designs.

With Single Molecule, Real-Time (SMRT®) sequencing on the Sequel® IIe systems, you can sequence AAV genome populations to identify truncation, mutation, and host integration events. The AAV workflow from PacBio® accommodates both scAAV and ssAAV constructs with easy on-instrument HiFi read generation

High-quality HiFi reads detect a wide range of cancer-specific somatic variation including single nucleotide variants (SNVs), structural variants (SVs), insertions and deletions (indels), copy number variations (CNVs), and methylation in a single assay. HiFi sequencing shows improved somatic variant calling accuracy with less sequencing relative to other technologies.

This Application note provides the workflow for the detection of somatic small variants, structural variants, and methylation for paired tumor-normal samples with HiFi whole genome sequencing (WGS)

Kinnex offers full-length isoform discovery and abundance information, cell type-specific isoform discovery, and species-level metagenomics community identification that are difficult to capture with short reads.

SMRT® Link software tools for PacBio® HiFi sequencing systems are designed to set up and monitor sequencing runs, review performance metrics, and analyze, visualize, and annotate sequencing data. SMRT Link is available for both local installation and cloud access, offering flexibility to work in different environments depending on your lab’s needs.