2024年05月30日 | RNA测序
Asset Type： Technical Note
2024年02月24日 | 样品准备
Technical note — Extracting HMW DNA from diverse insect samples using the Nanobind PanDNA kit for optimal HiFi sequencing
2023年11月17日 | procedures + protocols 产品、程序+实验方案
Technical note — Size selection of PacBio SMRTbell libraries with the Lightbench Instrument from Yourgene Health
2023年09月22日 | procedures + protocols 产品、程序+实验方案
Technical note — High throughput DNA shearing for HiFi whole genome sequencing from whole blood samples
2023年05月25日 | procedures + protocols 产品、程序+实验方案
Technical note – HT plate shearing microbial inserts
2022年06月30日 | 全基因组测序
HiFi WGS Performance with AMPure PB bead size selection
AMPure® PB bead size selection is a simple, quick, and scalable approach to deplete molecules less than 5 kb in a SMRTbell® library. For high-quality DNA, the sequencing performance and application metrics for assembly and variant calling are comparable to gel cassette size-selection methods.
2022年04月20日 | 样本+文库制备
Alternative Size Selection Methods for SMRTbell Prep Kit 3.0
PacBio recommends to use size-selection beads are to remove short DNA fragments and enrich for the long fragments that are most valuable for downstream analysis. Although size-selection beads have many advantages, alternative methods — like gels and DNA precipitation — offer more flexibility in defining a size cutoff. This technical note provides guidance for alternative methods of size selection of WGS libraries prepared with the SMRTbell prep kit 3.0.
2022年04月20日 | 样本+文库制备
Technical note – Covaris-g TUBE gDNA shearing for SMRTbell prep kit 3.0
PacBio recommends that DNA shearing for whole genome sequencing be performed on the Diagenode Megaruptor 3 system, which is the only system validated by PacBio for shearing, in order to produce consistent HiFi yield and quality. When a Megaruptor 3 system is unavailable, the Covaris g-TUBE presents an alternative shearing method that does not require instrumentation beyond a standard microcentrifuge. This Technical note provides guidelines for using the Covaris g-TUBE for DNA shearing.
2022年04月20日 | 人类遗传学研究
Technical note – Human whole blood sample prep
Sample preparation is a critical factor for whole genome sequencing (WGS) projects that impacts sequencing yield, read length, and ultimately performance for variant calling and genome assembly. Best practices to optimize performance for PacBio HiFi sequencing are to collect whole human blood, store with potassium EDTA as the anticoagulant for fewer than 2 days at 4°C, and extract DNA from 200 μL of blood using the Nanobind CBB Big DNA kit. When it is not possible to extract from fresh samples, storing samples at 4°C maximizes extraction yield and minimizes degradation for at least 9 days.
2021年08月19日 |
Technical Note: Preparing samples for PacBio whole genome sequencing for de novo assembly – Collection and storage
Single Molecule, Real-Time (SMRT) Sequencing uses the natural process of DNA replication to sequence long fragments of native DNA. As such, starting with high-quality, high molecular weight (HMW) genomic DNA (gDNA) will result in better sequencing performance across difficult to sequence regions of the genome. To obtain the highest quality, long DNA it is important to start with sample types compatible with HMW DNA extraction methods. This technical note is intended to give general guidance on sample collection, preparation, and storage across a range of commonly encountered sample types used for SMRT Sequencing whole genome projects. It is important to note that all samples and projects are unique and may not be comprehensively addressed in this document.
2021年08月19日 |
Technical Note: Preparing DNA for PacBio HiFi sequencing – Extraction and quality control
Single Molecule, Real-Time (SMRT) Sequencing uses the natural process of DNA replication to sequence long fragments of native DNA in order to produce highly accurate long reads, or HiFi reads. As such, starting with high-quality, high molecular weight (HMW) genomic DNA (gDNA) will result in longer libraries and better performance during sequencing. This technical note is intended to give recommendations, tips and tricks for the extraction of DNA, as well as assessing and preserving the quality and size of your DNA sample to be used for HiFi sequencing.
