19 4 月, 2024
Technical overview – Whole genome and metagenome library preparation using SMRTbell prep kit 3.0
- Tutorial
- Sequel II System:Sequel IIe System, Revio system
- Sample Preparation, Library Preparation, Binding, Sequencing, Analysis
This technical training presentation describes sample preparation details for constructing whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using SMRTbell prep kit 3.0 (SPK 3.0) for sequencing on PacBio Sequel II and Sequel IIe systems. Options for multiplexing WGS samples using SMRTbell barcoded adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations for de novo assembly, variant detection and shotgun metagenomics are also presented.