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Featured Documentation

This Featured Documentation page provides the latest and most frequently used guides, protocols, and product information. See Other Documentation for more specialized and older documentation.

4 1 月, 2024

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

  • Procedure & Checklist
  • Revio system, Sequel II System:Sequel IIe System
  • Library Preparation

Description

This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio® Revio™, Sequel® II, and IIe systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters.
11 4 月, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the Hamilton NIMBUS Presto system

  • Procedure & Checklist
  • Sequel II System:Sequel IIe System, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
11 4 月, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Apex system

  • Procedure & Checklist
  • Sequel II System:Sequel IIe System, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
11 4 月, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Duo Prime system

  • Procedure & Checklist
  • Sequel II System:Sequel IIe System, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
11 4 月, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Flex system

  • Procedure & Checklist
  • Sequel II System:Sequel IIe System, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Flex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
11 4 月, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the Hamilton NIMBUS Presto system

  • Procedure & Checklist
  • Sequel II System:Sequel IIe System, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
11 4 月, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Apex system

  • Procedure & Checklist
  • Revio system, Sequel II System:Sequel IIe System
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
11 4 月, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Duo Prime system

  • Procedure & Checklist
  • Sequel II System:Sequel IIe System, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This protocol requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
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