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Procedure & checklist – Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.

Procedure & checklist Preparing Onso libraries from existing P5P7 libraries for short-read sequencing

Description

This procedure describes the workflow for converting existing P5/P7-tailed libraries to be compatible for sequencing on PacBio short-read sequencing systems.

Procedure & checklist – Generating hybrid capture libraries for sequencing on the Onso system

Description

This procedure describes how the Onso DNA Library prep kit (PacBio 102-431-400), Onso Library amp kit (PacBio 102-410-800) and Onso Blocker oligos (PacBio 102-427-200) can be used to support the generation of hybrid capture libraries compatible with sequencing on PacBio short-read sequencing systems.

Procedure & checklist – Preparing Onso libraries from fragmented DNA for short-read sequencing

Description

This procedure describes the workflow for constructing Onso libraries from fragmented genomic material for sequencing on PacBio short-read sequencing systems.

Procedure & checklist – Preparing Onso libraries from genomic DNA for short read sequencing

Description

This procedure describes the workflow for constructing Onso libraries from high molecular weight genomic DNA for sequencing on PacBio short-read sequencing systems. This procedure produces PCR-free and/or PCR- amplified libraries for clustering.

Procedure & checklist — qPCR quantification of Onso libraries

Description

This procedure describes the workflow for the qPCR quantification of Onso libraries constructed for sequencing on PacBio short-read sequencing systems.

Procedure & checklist — Extracting HMW DNA using Nanobind HT 1 mL blood kit for mammalian whole blood on KingFisher Apex system

Procedure & checklist — Extracting HMW DNA using Nanobind HT 1 mL blood kit for mammalian whole blood on KingFisher Duo Prime system

Procedure & checklist — Extracting HMW DNA using Nanobind HT 1 mL blood kit for mammalian whole blood on KingFisher Flex system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for mammalian whole blood on Hamilton NIMBUS Presto system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 uL mammalian whole blood on Hamilton NIMBUS Presto system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 uL mammalian whole blood on KingFisher Apex system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 uL mammalian whole blood on KingFisher Duo Prime system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on Hamilton NIMBUS Presto system