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Procedure & checklist – Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Procedure & checklist — Generating PureTarget repeat expansion panel libraries

Description

This procedure describes the workflow for generating PureTarget repeat expansion libraries.

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters.

Procedure & checklist — Preparing whole genome libraries using the HiFi prep kit 96

Description

This procedure describes the high throughput workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96.

Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing

Description

This procedure describes the workflow for constructing SMRTbell libraries with PCR amplification from as little as 1 ng of starting genomic DNA using the Ampli-Fi protocol. Sequencing libraries prepared with this procedure are compatible with PacBio Revio and Vega systems.

Procedure & checklist – Preparing Onso libraries from genomic DNA for short read sequencing

Description

This procedure describes the workflow for constructing Onso libraries from high molecular weight genomic DNA for sequencing on PacBio short-read sequencing systems. This procedure produces PCR-free and/or PCR- amplified libraries for clustering.

Procedure & checklist – Preparing multiplexed AAV SMRTbell libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to prepare multiplexed AAV libraries for sequencing on the Sequel II and Sequel IIe systems. Multiplexing is achieved using SMRTbell barcoded adapters.

Procedure & checklist — Preparing Kinnex libraries from 16S rRNA amplicons

Description

This procedure provides instructions for generating Kinnex libraries from full-length 16S amplicons for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist – Extracting HMW DNA from animal tissue using the Nanobind PanDNA kit

Description

This procedure can be used to extract HMW DNA from animal tissues using the nanobind PanDNA kit.

Procedure & checklist – Extracting HMW DNA from plant nuclei using Nanobind kits

Description

This procedure can be used to extract HMW DNA from plant nuclei.

Procedure & checklist – Isolating nuclei from plant tissue using LN2 disruption

Description

This procedure can be used to isolate nuclei from plant tissues for HMW DNA extraction using the Nanobind plant nuclei kit

Procedure & checklist – Isolating nuclei from plant tissue using TissueRuptor disruption

Description

This procedure can be used to isolate nuclei from plant tissues for HMW DNA extraction using the Nanobind plant nuclei kit

Procedure & checklist — Extracting HMW DNA from insects using the Nanobind PanDNA kit

Description

This protocol describes the extraction of HMW (50–300+ kb) DNA from insects and other arthropods. It is recommended for PacBio® HiFi sequencing. This protocol requires the Nanobind PanDNA kit (103-260-000).

Procedure & checklist — Removing short DNA fragments with the Short Read Eliminator (SRE) kit

Description

This procedure describes the workflow for DNA size selection to remove molecules <10 kb using the SRE kit on high molecular weight (HMW) DNA before shearing and library preparation.

Procedure & checklist – Preparing Iso-Seq libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing Iso-Seq libraries using SMRTbell prep kit 3.0 from RNA for sequencing on PacBio HiFi systems