Featured Documentation

Procedure & checklist – Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Procedure & checklist – Generating PureTarget libraries with PureTarget kit 24 manual protocol

Description

This procedure describes the workflow for manually generating PureTarget libraries.

Procedure & checklist – Generating PureTarget libraries with PureTarget kit 96 automation protocol

Description

This procedure describes the workflow for generating PureTarget repeat expansion or carrier libraries.

Procedure & checklist – Preparing Iso-Seq libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing Iso-Seq libraries using SMRTbell prep kit 3.0 from RNA for sequencing on PacBio HiFi systems

Procedure & checklist – Preparing multiplexed AAV SMRTbell libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to prepare multiplexed AAV libraries for sequencing on the Sequel II and Sequel IIe systems. Multiplexing is achieved using SMRTbell barcoded adapters.

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters.

Procedure & Checklist – Preparing SMRTbell Libraries using PacBio Barcoded M13 Primers for Multiplex SMRT Sequencing

Description

This procedure can be used to generate asymmetrically barcoded PCR products for library construction and SMRT sequencing by amplifying the region of interest using target-specific PCR primers tailed with M13 forward and reverse primers.

Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing

Description

This procedure describes the workflow for constructing SMRTbell libraries with PCR amplification from as little as 1 ng of starting genomic DNA using the Ampli-Fi protocol. Sequencing libraries prepared with this procedure are compatible with PacBio Revio and Vega systems.

Procedure & checklist — Preparing Kinnex libraries from 16S rRNA amplicons

Description

This procedure provides instructions for generating Kinnex libraries from full-length 16S amplicons for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing Kinnex libraries using the Kinnex full-length RNA kit

Description

This procedure describes the workflow for constructing Kinnex full-length RNA libraries from total RNA samples for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing multiplexed whole genome and amplicon libraries using the HiFi plex prep kit 96

Description

This procedure describes the workflow for constructing multiplexed whole genome sequencing (WGS) and amplicon libraries using the HiFi plex prep kit 96 for sequencing on PacBio systems. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.

Procedure & checklist — Preparing whole genome libraries using the HiFi prep kit 96

Description

This procedure describes the high throughput workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96.

Procedure & checklist – Amplification of bacterial full-length 16S rRNA gene with barcoded primers

Description

This procedure describes the PCR amplification of bacterial full-length 16S rRNA genes (V1—V9 regions) using up to 192 dual indices.

Procedure & checklist — Generating PureTarget repeat expansion panel libraries

Description

This procedure describes the workflow for generating PureTarget repeat expansion libraries.