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Procedure & checklist – Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Guide — Vega system site preparation

Description

Guide for Vega system site preparation

Safety guide – Vega system

Description

Safety guide for the vega system

Operations guide – Revio system

Description

This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT Link (v12.0) user guide.

Operations guide – Vega system

Description

Operations guide for the Vega system

SMRT Link User Guide v13.1

Description

This document describes how to use SMRT Link software from PacBio. SMRT Link is the web-based end-to-end workflow manager for Revio, Sequel II, and Sequel IIe systems.

Release notes — Revio System instrument control software v13.3

Description

Release notes - Revio System instrument control software v13.3

SMRT Link Release Notes v25.1

Description

SMRT Link Release Notes v25.1

SMRT Link Release Notes v25.2

Description

SMRT Link release notes for v25.2

Procedure & checklist – Preparing Iso-Seq libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing Iso-Seq libraries using SMRTbell prep kit 3.0 from RNA for sequencing on PacBio HiFi systems

Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing

Description

This procedure describes the workflow for constructing SMRTbell libraries with PCR amplification from as little as 1 ng of starting genomic DNA using the Ampli-Fi protocol. Sequencing libraries prepared with this procedure are compatible with PacBio Revio and Vega systems.

Procedure & checklist — Generating PureTarget repeat expansion panel libraries

Description

This procedure describes the workflow for generating PureTarget repeat expansion libraries.

Procedure & checklist — Preparing Kinnex libraries from 16S rRNA amplicons

Description

This procedure provides instructions for generating Kinnex libraries from full-length 16S amplicons for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing Kinnex libraries using the Kinnex full-length RNA kit

Description

This procedure describes the workflow for constructing Kinnex full-length RNA libraries from total RNA samples for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.