Featured Documentation

Operations guide – Revio system

Description

This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT Link (v12.0) user guide.

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters.

Procedure & checklist – Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Guide — Vega system site preparation

Description

Guide for Vega system site preparation

Release notes — Revio system instrument control software v13.1

Description

Release notes for the Revio system Instrument Control Software v13.1

SMRT Link User Guide v13.1

Description

This document describes how to use SMRT Link software from PacBio. SMRT Link is the web-based end-to-end workflow manager for Revio, Sequel II, and Sequel IIe systems.

SMRT Link User Guide v13.0

Description

This document describes how to use SMRT Link software from PacBio. SMRT Link is the web-based end-to-end workflow manager for Revio, Sequel II, and Sequel IIe systems.

Overview – HiFi application options

Description

Overview of HiFi application options

Guide & overview — Automated HiFi prep 96 and HiFi annealing binding and cleanup for the Hamilton NGS Microlab STAR system

Description

This Guide & Overview describes the high throughput automated workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96 on the Hamilton NGS Microlab STAR MOA system.

Guide & overview — Automated HiFi plex prep 96 for the Hamilton NGS Microlab STAR system

Description

This Guide & Overview describes the workflow for PacBio high-throughput semi-automated long read sample prep utilizing the HiFi plex prep 96 kit on the Hamilton NGS Microlab STAR MOA system. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.

Guide & overview — Short Read Eliminator SRE DNA shearing and cleanup for the Hamilton Microlab Prep system

Description

This Guide & overview describes the sample, reagent, and consumable preparation for Short Read Eliminator (SRE), DNA shearing, and post-shear cleanup on the Hamilton Microlab Prep (MLP) Liquid Handling system. This Guide & overview is meant to be used with the HiFi prep kit 96, HiFi plex prep kit 96, or SMRTbell® prep kit 3.0 library protocols.

Procedure & checklist – Preparing Iso-Seq libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing Iso-Seq libraries using SMRTbell prep kit 3.0 from RNA for sequencing on PacBio HiFi systems

Procedure & checklist — Generating PureTarget repeat expansion panel libraries

Description

This procedure describes the workflow for generating PureTarget repeat expansion libraries.

Procedure & checklist — Preparing Kinnex libraries from 16S rRNA amplicons

Description

This procedure provides instructions for generating Kinnex libraries from full-length 16S amplicons for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.