Detection of low-frequency somatic variants using Single-Molecule, Real-Time Sequencing

Author(s): Baybayan, P. and Hall, R. and Lambert, C. and Chakroborty, S. and Guo, Y. and Huang, C. and Anakella, B.i and Kujawa, S.

Detection of somatic mutations, especially in heterogeneous tumor samples where variants may be present at a low level, is challenging. Single Molecule, Real-Time (SMRT) Sequencing is ideal for minor variant detection because of its ability to sequence single molecules with very high accuracy (>QV40) using the circular consensus sequencing (CCS) approach.

Organization: PacBio
Year: 2017

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