Menu
2021年06月01日

Applying Sequel to Genomic Datasets

Author(s): Clum, Alicia and Daum, Chris and Kotturi, Paul and LaButti, Kurt and O'Malley, Ronan and Pangilinan, Jasmyn and Spittle, Kristi and Zane, Matt and Copeland, Alex

De novo assembly is a large part of JGI’s analysis portfolio. Repetitive DNA sequences are abundant in a wide range of organisms we sequence and pose a significant technical challenge for assembly. We are interested in long read technologies capable of spanning genomic repeats to produce better assemblies. We currently have three RS II and two Sequel PacBio machines. RS II machines are primarily used for fungal and microbial genome assembly as well as synthetic biology validation. Between microbes and fungi we produce hundreds of PacBio libraries a year and for throughput reasons the vast majority of these are >10 kb AMPure libraries. Throughput for RS II is about 1 Gb per SMRT Cell. This is ideal for microbial sized genomes but can be costly and labor intensive for larger projects which require multiple cells. JGI was an early access site for Sequel and began testing with real samples in January 2016. During that time we’ve had the opportunity to sequence microbes, fungi, metagenomes, and plants. Here we present our experience over the last 18 months using the Sequel platform and provide comparisons with RS II results.

Organization: Joint Genome Institute
Year: 2017

View Conference Poster

咨询专家

如果您有疑问、需要查看订单状态或想要购买仪器,我们随时乐意提供帮助。

姓名(Required)
这个字段是用于验证目的,应该保持不变。

在本网页上注册,即表示您同意,并同意 PacBio 根据我们的隐私政策收集和使用该信息.