Resolving KIR genotypes and haplotypes simultaneously using Single Molecule, Real-Time Sequencing

Author(s): Ekholm, JM and Pyo, CW and Eng, K and Hall, R, and Vierra-Green, C and Spellman, S and Roe, D and Geraghty, DE

The killer immunoglobulin-like receptors (KIR) genes belong to the immunoglobulin superfamily and are widely studied due to the critical role they play in coordinating the innate immune response to infection and disease. Highly accurate, contiguous, long reads, like those generated by SMRT Sequencing, when combined with target-enrichment protocols, provide a straightforward strategy for generating complete de novo assembled KIR haplotypes. We have explored two different methods to capture the KIR region; one applying the use of fosmid clones and one using Nimblegen capture.

Organization: PacBio
Year: 2016

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